Image of Selection and characterization of potential bacteria for polycyclic aromatic biodegradation of hydrocarbons in sea sponges from Spermonde Islands, Indonesia

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Selection and characterization of potential bacteria for polycyclic aromatic biodegradation of hydrocarbons in sea sponges from Spermonde Islands, Indonesia

Some mucous substances that cover the sponge body surface are related to the degradation ability of symbiotic bacteria of poly aromatic hydrocarbons (PAHs). This study aims to demonstrate the above statement through a morphological, phenotypical, and genotypic investigation of the bacterial symbionts. The following tracing methods were used in this study: morphological analysis, phenotype analysis (Gram staining and biochemical tests), microsymbiont genotyping using PCR (Polymerase chain reaction), and growth test activity of symbiont isolates on PAH contaminated solid and liquid media (anthracene, pyrene and mix 16 PAH ASTM standard). Three types of sponge symbiont isolate, Pseudomonas stutzeri RCH2, Bacillus licheniformis ATCC9789, and Bacillus sp. AB353 partially showed growth activity on both types of test media (solid and liquid media), whereas Acinotobacter calcoaceticus PHKDB14 showed no growth activity. These results indicate that there are morphological, Gram grouping, phenotypic characteristics, and bacterial symbiont genotypes correlation with PAH degradation ability.

Ketersediaan
UCM-P-J-0007-1UCM-P-J-0007My LibraryTersedia
Informasi Detail
Judul Seri
Vol. 13 No. 6 (2020)
No. Panggil
UCM-P-J-0007
Penerbit
: Bioflux Publishing House.,
Deskripsi Fisik
-
Bahasa
English
ISBN/ISSN
1844-8143
Klasifikasi
Jurnal
Tipe Isi
text
Tipe Media
computer
Tipe Pembawa
online resource
Edisi
Vol. 13 No. 6 (2020)
Subjek
Sponges
Info Detail Spesifik
Bacterial symbionts, degradation, PAH, sponge
Pernyataan Tanggungjawab
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